SPR optics

During the biomolecular interaction process the instrument is filming and grabbing the images of the sensor surface. An SPR-reflectivity image can be taken for locating/positioning the regions of interests (RoI) and to inspect the quality of the sensor surface. A sensor surface area of 6.6 by 8.8 mm is imaged. The sensitivity of the IBIS-MX48 has been improved dramatically and is better than 0.02 millidegree or 0.2 RU (RMSD).

In fixed-angle instruments the applicable range is limited and spot to spot sensitivity differ considerably when ligand or analyte panels having different molecular weights are to be monitored. The use of only one fixed incident light angle is therefore not suitable for the monitoring of multiple biomolecular interactions in parallel on a microarray. The scanning angle principle in the IBIS instrument overcomes spot to spot sensitivity problems and linearity issues.