| General |
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|
| Dimensions (DxWxH) |
|
515 x 620 x 500 mm |
| Weight |
|
65 kg |
| Electrical supply |
|
100-240 V (60-50 Hz) |
| SPR Configuration |
|
Kretschmann |
| Optics |
|
scanning angle |
| Imaging |
|
area 8.8 x 6.6 mm (1600 x 1200 pixels) |
| Optical lateral resolution |
|
25 µm |
| Maximum number of ROIs |
|
> 500 (typically 2 x 48 spots -CFM) |
| SPR control |
|
template (embedded settings) |
| Angle range |
|
10 degrees, corresponds to ~1.33-1.43 refractive index units (RIU) |
| Dynamic angle range |
|
3 degrees, corresponds to ~1.33-1.36 RIU |
Temperature control
Temperature stability flow cell |
|
Independent for both sample deck and flow-cell
< 0.01 °C |
| Analysis temperature |
|
15-45°C (7°C below ambient) |
| Typical working ranges |
|
|
| Affinity constant (KD equilibrium) |
|
10-6; - 10-12 M |
| Baseline noise (typical) |
|
<0.02 mo (RMSD for 100x100 RoI) |
| Baseline stability |
|
<0.08 mo/min |
| Overnight run samples / sensorgrams |
|
96 samples / ~5000 sensorgrams |
| |
|
|
| Liquid handling |
|
|
| Sample capacity |
|
96 well plate, 12 vials (1.5 ml) 3 containers (10 ml) |
| Syringes and volume |
|
2 syringes, 1000 µl/stroke |
| Required sample volume |
|
100 µl |
| Injection Volume |
|
70 µl |
Flow cell volume
Flow cell area |
|
12 µl
6 x 10 mm (2x 48 spots CFM) |
| Injection rise time to steady |
|
< 2 seconds |
| Scripts |
|
embedded library in templates |
| |
|
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| Software |
|
|
| Operating system and program |
|
MS Windows XP SP3, Tri-angle data acquisition |
| Kinetic evaluation for sample and RoI analysis |
|
SPRint, cincluding export functions to MS Excel, Scrubber, txt files |
| Data management |
|
User mode, templates personal, protected |
| Data analysis |
|
SPRint software package, serial, overlay, calibration, tiled plots, presentation of sensorgrams of analytes and RoI's independently, zero, crop, align, reference, R, slope and kd rate constant, 3D, smooth, despiking. |
| Common mode subtraction |
|
yes, direct compensation of bulk refractive index shifts, temperature effects. |