The Instrument for Biomolecular Interaction Sensing MultipleX 96 (IBIS-MX96) is an array-based SPR imaging system for multiplex biomolecular interaction analysis.
The MX96 is optimized for monitoring 96 ligand spots on a sensor surface, which are deposited from flow with the CFM Printer. Using the MX96’s temperature controlled 96 well autosampler, analytes are injected in series across the chip, yielding 96 binding interactions for each injection. It is possible to perform unattended overnight or weekend runs from a 96 well plate, thereby generating up to 10,000 sensorgrams in a full run, including controls.
The MX96 utilizes a proprietary back-and-forth flow for sample injection, enabling long association times with only 100 µL of sample!
By combining the MX96 with the CFM, standard biosensor protocols can be transferred from lower throughput machines to the higher-throughput array system. For applications where multiplexing is important the array can be extremely powerful, because sample consumption and instrument run times can scale rapidly on other platforms as the experiments grow in size. For example, the array enables pairwise competition of 96 mAbs – almost 10,000 individual binding interactions – using only 200 µL of each mAb and a single 24 hour run. This could take days to months on other platforms and many milliliters of each mAb.